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mouse anti mical l1 (Novus Biologicals)

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Novus Biologicals mouse anti mical l1
Mouse Anti Mical L1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h00085377/pmc11790967-37-0-5?v=Novus+Biologicals
Average 91 stars, based on 23 article reviews

mouse anti mical l1 - by Bioz Stars, 2026-08

91/100 stars

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mouse polyclonal anti mical l1 antibody (Novus Biologicals)

Novus Biologicals mouse polyclonal anti mical l1 antibody

Hela cells grown on coverslips were serum starved for 2 hours, treated either with 10μg/ml of transferrin (TFN)) or BSA, kept at 4°C for one hour and incubated at 37°C for indicated time. The cells were then fixed, stained for endogenous Rab8 and <t>MICAL-L1</t> and examined by confocal microscope. Representative confocal images after 20 minutes of treatment with BSA and transferrin are shown (A). Bar diagrams show quantitation of the percentage of cells exhibiting RAB8 tubules (B), or MICAL-L1 tubules (C) at indicated time. Data represent mean ±s.d. from 3 independent experiments done with 265-332 cells. High resolution images show colocalization of Rab8 tubules with MICAL-L1 tubules (D). Scale bar, 10 μm.

Mouse Polyclonal Anti Mical L1 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h00085377/bio_rxiv__2023__02__06__527405-51-21-27?v=Novus+Biologicals
Average 94 stars, based on 1 article reviews

mouse polyclonal anti mical l1 antibody - by Bioz Stars, 2026-08

94/100 stars

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Image Search Results

Journal: The EMBO Journal

Article Title: A novel human fetal lung-derived alveolar organoid model reveals mechanisms of surfactant protein C maturation relevant to interstitial lung disease

doi: 10.1038/s44318-024-00328-6

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Mouse anti-Mical-L1 , 1:100 , Novus , H00085377.

Techniques: Recombinant, Transduction, CRISPR, Gene Knockout, Expressing, Concentration Assay, Immunofluorescence, Western Blot, Flow Cytometry, Sequencing, Red Blood Cell Lysis, Cell Recovery, Plasmid Preparation, SYBR Green Assay, Reverse Transcription, Software, Microscopy, Magnetic Beads, Transmission Assay

Journal: bioRxiv

Article Title: Transferrin-induced signaling through transferrin receptor and AKT kinase mediates formation of Rab8- and MICAL-L1-positive tubules involved in receptor recycling

doi: 10.1101/2023.02.06.527405

Figure Lengend Snippet: Hela cells grown on coverslips were serum starved for 2 hours, treated either with 10μg/ml of transferrin (TFN)) or BSA, kept at 4°C for one hour and incubated at 37°C for indicated time. The cells were then fixed, stained for endogenous Rab8 and MICAL-L1 and examined by confocal microscope. Representative confocal images after 20 minutes of treatment with BSA and transferrin are shown (A). Bar diagrams show quantitation of the percentage of cells exhibiting RAB8 tubules (B), or MICAL-L1 tubules (C) at indicated time. Data represent mean ±s.d. from 3 independent experiments done with 265-332 cells. High resolution images show colocalization of Rab8 tubules with MICAL-L1 tubules (D). Scale bar, 10 μm.

Article Snippet: Mouse monoclonal anti-RAB8 antibody was from BD Biosciences (#610845) (San Jose, CA, USA); rabbit polyclonal anti-RAB8 antibody was from Sigma (#R5530); mouse polyclonal anti-MICAL-L1 antibody was from Novus Biologicals (H00085377-B01P); rabbit polyclonal anti-Akt antibody was from Cell signalling (#9272); mouse monoclonal anti-transferrin receptor antibody was from Calbiochem (#GRO9L); anti-mouse and anti-rabbit Cy3 conjugated secondary antibodies were from Amersham; anti-rabbit and anti-mouse Alexafluor-488 conjugated secondary antibodies, Alexa Fluor 546-labelled transferrin (#T23364) were from Molecular probes; Akt inhibitor AI4 was from Calbiochem (#124011); Src family kinase inhibitor SU6656 was from Sigma (##9692); Wortmannin (phosphatidylinositol 3-kinase inhibitor) was from Calbiochem (#681675); bovine serum albumin (BSA) (# A9418) and unlabelled human holo-transferrin (#T0665) were from Sigma.

Techniques: Incubation, Staining, Microscopy, Quantitation Assay

Hela cells grown on coverslips were serum starved for 2 hours, preincubated with TFRC blocking antibody or control IgG for 30 minutes and then treated with either transferrin or BSA, kept at 4°C for 1 hour followed by incubation at 37°C for 20 minutes. The cells were then fixed, stained for endogenous RAB8 and MICAL-L1 and examined by confocal microscope. Representative confocal images are shown (A). Bar diagrams show quantitation of the percentage of cells exhibiting MICAL-L1 tubules (B), or RAB8 tubules (C). Data represent mean± s.d. from 3 experiments done with 298-349 cells. *** P<0.0001, ** P<0.01. Scale bar, 10 μm.

Journal: bioRxiv

Article Title: Transferrin-induced signaling through transferrin receptor and AKT kinase mediates formation of Rab8- and MICAL-L1-positive tubules involved in receptor recycling

doi: 10.1101/2023.02.06.527405

Figure Lengend Snippet: Hela cells grown on coverslips were serum starved for 2 hours, preincubated with TFRC blocking antibody or control IgG for 30 minutes and then treated with either transferrin or BSA, kept at 4°C for 1 hour followed by incubation at 37°C for 20 minutes. The cells were then fixed, stained for endogenous RAB8 and MICAL-L1 and examined by confocal microscope. Representative confocal images are shown (A). Bar diagrams show quantitation of the percentage of cells exhibiting MICAL-L1 tubules (B), or RAB8 tubules (C). Data represent mean± s.d. from 3 experiments done with 298-349 cells. *** P<0.0001, ** P<0.01. Scale bar, 10 μm.

Techniques: Blocking Assay, Control, Incubation, Staining, Microscopy, Quantitation Assay

Hela cells grown on coverslips were serum starved for 2 hours, preincubated with Dynasore (80μM) or DMSO (0.1%) for 30 minutes and then treated with either transferrin or BSA, kept at 4°C for 1 hour followed by incubation at 37°C for 20 minutes. The cells were then fixed, stained for endogenous RAB8 and MICAL-L1, and examined by confocal microscope. Representative confocal images are shown (A) Scale bar: 10μm. Bar diagrams show quantitation of the percentage of cells exhibiting MICAL-L1 tubules (B), or RAB8 tubules (C). Data represent mean± s.d. from 3 experiments done with 328-347 cells. *** P<0.0001, ** P<0.01.

Journal: bioRxiv

Article Title: Transferrin-induced signaling through transferrin receptor and AKT kinase mediates formation of Rab8- and MICAL-L1-positive tubules involved in receptor recycling

doi: 10.1101/2023.02.06.527405

Figure Lengend Snippet: Hela cells grown on coverslips were serum starved for 2 hours, preincubated with Dynasore (80μM) or DMSO (0.1%) for 30 minutes and then treated with either transferrin or BSA, kept at 4°C for 1 hour followed by incubation at 37°C for 20 minutes. The cells were then fixed, stained for endogenous RAB8 and MICAL-L1, and examined by confocal microscope. Representative confocal images are shown (A) Scale bar: 10μm. Bar diagrams show quantitation of the percentage of cells exhibiting MICAL-L1 tubules (B), or RAB8 tubules (C). Data represent mean± s.d. from 3 experiments done with 328-347 cells. *** P<0.0001, ** P<0.01.

Techniques: Incubation, Staining, Microscopy, Quantitation Assay

Hela cells grown on coverslips were serum starved for 2 hours, and treated with either BSA or transferrin and with DMSO (0.05%) or Akt inhibitor AI-4, kept at 4°C for 1 hour followed by incubation at 37°C for 20 minutes. Cells were fixed and stained for endogenous RAB8 and MICAL-L1 and observed by confocal microscope. Representative confocal images are shown (A) Scale bar: 10 μm. Bar diagrams show quantitation of the percentage of cells exhibiting MICAL-L1-positive (B) or RAB8-positive (C) tubules in each scoring category. Data from three separate experiments done with 411-503 cells are shown as the mean± s.d.***P<0.0001, *P<0.05. (D, E, F) Hela cells were transfected with dominant negative AKT. After 22 hours the cells were serum starved for 2 hours, treated with either BSA or transferrin, kept at 4°C for 1 hour followed by incubation at 37°C for 20 minutes. Cells were fixed and stained for endogenous RAB8 or MICAL-L1 and observed by confocal microscope. Representative confocal images are shown (D, F). Scale bar: 10 μm. Bar diagrams show percentage of cells exhibiting MICAL-L1 positive tubules (E) (transfected (T): 154cells; untransfected (UT): 602-608cells) or RAB8-positive tubules (G) (transfected: 99-119cells; untransfected: 360-443cells) in each scoring category. Data from three separate experiments are shown as the mean±s.d., ***P<0.0001, **P<0.01.

Journal: bioRxiv

Article Title: Transferrin-induced signaling through transferrin receptor and AKT kinase mediates formation of Rab8- and MICAL-L1-positive tubules involved in receptor recycling

doi: 10.1101/2023.02.06.527405

Figure Lengend Snippet: Hela cells grown on coverslips were serum starved for 2 hours, and treated with either BSA or transferrin and with DMSO (0.05%) or Akt inhibitor AI-4, kept at 4°C for 1 hour followed by incubation at 37°C for 20 minutes. Cells were fixed and stained for endogenous RAB8 and MICAL-L1 and observed by confocal microscope. Representative confocal images are shown (A) Scale bar: 10 μm. Bar diagrams show quantitation of the percentage of cells exhibiting MICAL-L1-positive (B) or RAB8-positive (C) tubules in each scoring category. Data from three separate experiments done with 411-503 cells are shown as the mean± s.d.***P<0.0001, *P<0.05. (D, E, F) Hela cells were transfected with dominant negative AKT. After 22 hours the cells were serum starved for 2 hours, treated with either BSA or transferrin, kept at 4°C for 1 hour followed by incubation at 37°C for 20 minutes. Cells were fixed and stained for endogenous RAB8 or MICAL-L1 and observed by confocal microscope. Representative confocal images are shown (D, F). Scale bar: 10 μm. Bar diagrams show percentage of cells exhibiting MICAL-L1 positive tubules (E) (transfected (T): 154cells; untransfected (UT): 602-608cells) or RAB8-positive tubules (G) (transfected: 99-119cells; untransfected: 360-443cells) in each scoring category. Data from three separate experiments are shown as the mean±s.d., ***P<0.0001, **P<0.01.

Techniques: Incubation, Staining, Microscopy, Quantitation Assay, Transfection, Dominant Negative Mutation

(A) Hela cells transfected with TBC1D17 or its catalytically inactive R381A mutant were stained for endogenous MICAL-L1. Confocal microscopy revealed that expression of TBC1D17 led to reduction in cells showing MICAL-L1 tubules. Scale bar: 10 μm. (B) The graph shows the percentage of cells exhibiting MICAL-L1-positive tubules in each scoring category. Data from three separate experiments are shown as the mean ± s.d. ***P<0.001.

Journal: bioRxiv

Article Title: Transferrin-induced signaling through transferrin receptor and AKT kinase mediates formation of Rab8- and MICAL-L1-positive tubules involved in receptor recycling

doi: 10.1101/2023.02.06.527405

Figure Lengend Snippet: (A) Hela cells transfected with TBC1D17 or its catalytically inactive R381A mutant were stained for endogenous MICAL-L1. Confocal microscopy revealed that expression of TBC1D17 led to reduction in cells showing MICAL-L1 tubules. Scale bar: 10 μm. (B) The graph shows the percentage of cells exhibiting MICAL-L1-positive tubules in each scoring category. Data from three separate experiments are shown as the mean ± s.d. ***P<0.001.

Techniques: Transfection, Mutagenesis, Staining, Confocal Microscopy, Expressing

HeLa cells grown on coverslips were serum starved for 2 hours, treated with BSA or transferrin as well as with DMSO (0.1%) or SU6656(Src kinase inhibitor10uM) or Wortmanin (250nM), kept at 4°C for 1 hour followed by incubation at 37°C for 20 minutes. Cells were fixed and stained for endogenous RAB8 and MICAL-L1 and observed by confocal microscope. Representative confocal images are shown (A) Scale bar: 10 μm. Bar diagrams show percentage of cells exhibiting MICAL-L1-positive (B) or RAB8-positive (C) tubules in each scoring category. Data from two separate experiments done with 100-165 cells are shown.

Journal: bioRxiv

Article Title: Transferrin-induced signaling through transferrin receptor and AKT kinase mediates formation of Rab8- and MICAL-L1-positive tubules involved in receptor recycling

doi: 10.1101/2023.02.06.527405

Figure Lengend Snippet: HeLa cells grown on coverslips were serum starved for 2 hours, treated with BSA or transferrin as well as with DMSO (0.1%) or SU6656(Src kinase inhibitor10uM) or Wortmanin (250nM), kept at 4°C for 1 hour followed by incubation at 37°C for 20 minutes. Cells were fixed and stained for endogenous RAB8 and MICAL-L1 and observed by confocal microscope. Representative confocal images are shown (A) Scale bar: 10 μm. Bar diagrams show percentage of cells exhibiting MICAL-L1-positive (B) or RAB8-positive (C) tubules in each scoring category. Data from two separate experiments done with 100-165 cells are shown.

Techniques: Incubation, Staining, Microscopy

Binding of transferrin to its receptor TFRC, a transmembrane protein leads to endocytosis. Activation of SRC upon binding of transferrin to its receptor has been reported earlier . Activated SRC leads to AKT activation, which then inactivates TBC1D17 (a GAP for RAB8) possibly through phosphorylation at S366. This results in RAB8 activation which is recruited to the tubules. Activated RAB8 facilitates recruitment of MICAL-L1 to the tubules. These tubules mediate recycling of transferrin-TFRC complex to the plasma membrane.

Journal: bioRxiv

Article Title: Transferrin-induced signaling through transferrin receptor and AKT kinase mediates formation of Rab8- and MICAL-L1-positive tubules involved in receptor recycling

doi: 10.1101/2023.02.06.527405

Figure Lengend Snippet: Binding of transferrin to its receptor TFRC, a transmembrane protein leads to endocytosis. Activation of SRC upon binding of transferrin to its receptor has been reported earlier . Activated SRC leads to AKT activation, which then inactivates TBC1D17 (a GAP for RAB8) possibly through phosphorylation at S366. This results in RAB8 activation which is recruited to the tubules. Activated RAB8 facilitates recruitment of MICAL-L1 to the tubules. These tubules mediate recycling of transferrin-TFRC complex to the plasma membrane.

Techniques: Binding Assay, Activation Assay, Phospho-proteomics, Clinical Proteomics, Membrane